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Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

DOI :

10.3791/55507-v

April 18th, 2017

April 18th, 2017

11,202 Views

1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus (UCAMC), 2Neuroscience Graduate Program, University of Colorado Anschutz Medical Campus (UCAMC)

This manuscript describes methods for electrophysiological recordings from spinal neurons of zebrafish embryos and larvae. The preparation maintains neurons in situ and often involves minimum dissection. These methods allow for the electrophysiological study of a variety of spinal neurons, from the initial electrical excitability acquisition through the early larval stages.

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Zebrafish

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