JoVE Logo
Faculty Resource Center

Sign In

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

DOI :

10.3791/50259-v

November 13th, 2013

November 13th, 2013

11,461 Views

1Departments of Pediatrics and Neurology, University of Michigan

Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.

Tags

Zebrafish

-- Views

Related Videos

article

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

article

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis

article

Microdissection of Zebrafish Embryonic Eye Tissues

article

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

article

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

article

Immunostaining of Dissected Zebrafish Embryonic Heart

article

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

article

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

article

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved