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Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

DOI :

10.3791/50259-v

November 13th, 2013

November 13th, 2013

11,362 Views

1Departments of Pediatrics and Neurology, University of Michigan

Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.

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