Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
6.7K Views
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10:18 min
•
June 26th, 2017
DOI :
June 26th, 2017
•1Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, 2Department of Neuroscience; Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine, 3Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, 4Department of BioSciences, Università degli Studi di Milano
Chapters in this video
0:05
Title
1:07
pHuC_Mermaid Plasmid Generation
3:54
Embryo Microinjection and Spontaneous Coiling Analysis
5:50
Imaging Setup for Mermaid Biosensor Visualization in Living Embryos: Simulataneous Detection of Donor and Acceptor Signals
8:23
Results: In Vivo FRET Analysis to Measure Changes in Motor Neuron Membrane Potential
9:39
Conclusion
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