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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

DOI :

10.3791/2384-v

9:13 min

November 11th, 2010

November 11th, 2010

13,291 Views

1Department of Neurobiology and Behavior, University of California, Irvine

Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.

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Cryopreservation

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