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Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

DOI :

10.3791/55396-v

February 15th, 2017

February 15th, 2017

11,315 Views

1The MARCS Institute, Western Sydney University, 2School of Medicine, Western Sydney University

Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.

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Prolonged Incubation

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