February 23rd, 2011
•Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
Tags
Related Videos
Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila
Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration
Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
Isolating Nasal Olfactory Stem Cells from Rodents or Humans
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved