JoVE Logo

Sign In

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

8.4K Views

14:02 min

April 9th, 2018

DOI :

10.3791/57069-v

April 9th, 2018


Explore More Videos

GPCR

Chapters in this video

0:04

Title

1:11

Fluorescence-based Screening of Incorporation Efficiencies

4:22

Genetic Incorporation of ncAAs into GPCRs

8:21

Ultrafast Bioorthogonal Labeling of GPCRs on Live Mammalian Cells

11:11

Results: Incorporation of Chemical Probes into GPCRs in Mammalian Cells for Cross-linking and Imaging Studies

12:52

Conclusion

Related Videos

article

10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

12.5K Views

article

14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

10.7K Views

article

07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

7.6K Views

article

08:21

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

6.8K Views

article

14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

5.0K Views

article

08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

2.1K Views

article

14:26

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

13.2K Views

article

09:40

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

17.9K Views

article

09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

2.3K Views

article

16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

15.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved