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Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor Video (Video) | JoVE

DOI :

10.3791/52652-v

July 13th, 2015

July 13th, 2015

26,444 Views

1UMR Centre des Sciences du Goŭt et de l'Alimentation, CNRS, INRA, Université de Bourgogne

Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.

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