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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

34.8K Views

09:24 min

July 29th, 2007

DOI :

10.3791/247-v

July 29th, 2007


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Qiagen Miniprep Kit

Chapters in this video

0:07

Introduction

0:46

Growing Up Bacteria

1:24

Resuspending and Lysing Bacteria with Buffers P1 and P2

3:56

Precipitating Bacterial Protein with Buffer N3

4:38

Assembling the Qiavac Manifold during Centrifugation

5:15

Binding Plasmid DNA to Qiastrip and Washing with Buffers PB and PE

7:19

Eluting DNA in Collection Tubes

8:48

Conclusion

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