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Whole Cell Recordings from Brain of Adult Drosophila

16.4K Views

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09:27 min

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July 29th, 2007

DOI :

10.3791/248-v

July 29th, 2007

•
1Department of Development and Cell Biology, Department of Anatomy and Neurobiology, University of California, Irvine (UCI)

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Whole Cell Recordings
Brain
Adult Drosophila
Procedure
Single Neurons
Dissecting Solution
Capture
Optic Lobes
Trachea
Isolated Brain
Neurons
Handling
Damage Prevention
Holder
Recording Chamber
Electrophysiology Setup
GAL4 Line
GFP Expression
Projection Neurons PNs
Live Brain
Nomarski Image
Targeted Neuron
Spontaneous Activity
Action Potentials
Synaptic Input
In Situ Preparation

Chapters in this video

0:04

Title

0:22

Introduction

0:53

Preparation of Dissection Solution

2:15

Adult Drosophila Selection

2:32

Sex Differentiation

3:04

Decapitation and Removal of Brain

4:19

Removal of Trachea and Enzyme Treatment

5:27

Stabilizing Brain in Recording Chamber

7:58

Conclusion

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