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Loading Drosophila Nerve Terminals with Calcium Indicators

DOI :

10.3791/250-v

July 30th, 2007

July 30th, 2007

9,971 Views

1Department of Physiology, University of Texas Health Science Center at San Antonio (UTHSCSA)

Calcium is a ubiquitous messenger in the nervous system, essential for triggering neurotransmitter release and changes in synaptic strength. Here we demonstrate a technique for loading Ca2+-indicators into Drosophila nerve terminals. We also demonstrate fabrication of the required apparatus and emphasize points critical for the technique's success.

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Drosophila

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