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Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

DOI :

10.3791/1154-v

May 13th, 2009

May 13th, 2009

10,658 Views

1Department of Zoology, University of British Columbia - UBC

We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans.

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