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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

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04:54 min

April 19th, 2011

April 19th, 2011

40,252 Views

0:05

Title

0:55

Making Protoplasts

2:14

Transformation of DNA into Protoplasts

4:30

Conclusion

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation1.

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