Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

4.1K Views

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07:50 min

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March 17th, 2023

DOI :

10.3791/64777-v

March 17th, 2023

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Hannah Levengood¹, Yanxia Dou¹, Jinping Fan¹, Anna Bajszar², Jing Huang¹, Syed Mohsin Abbas³, Yun Zhou⁴, Cankui Zhang¹

¹Department of Agronomy, Center for Plant Biology, Purdue University, ²Department of Biology, Purdue University, ³Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, ⁴Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

Summary

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Agrobacterium Tumefaciens

Genetic Transformation

Narrowleaf Plantain

Plantago Species

Vascular Biology

Stress Physiology

Transformation Protocol

Germination Process

Bacteria Colony

Plasmid Inoculation

Selection Agent

Chapters in this video

0:04

Introduction

0:32

Plant Material Propagation for Transformation

2:05

Agrobacterium tumefaciens Preparation

3:34

Transformation of Plantago Roots

4:55

Selection and Whole Plant Regeneration

5:38

Soil Transfer

6:09

Results: Agrobacterium tumefaciens-Mediated Genetic Transformation

7:25

Conclusion

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