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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

DOI :

10.3791/64777-v

7:50 min

March 17th, 2023

March 17th, 2023

2,665 Views

1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

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Keywords Agrobacterium Tumefaciens

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