JoVE Logo
Faculty Resource Center

Sign In

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

DOI :

10.3791/64777-v

7:50 min

March 17th, 2023

March 17th, 2023

1,858 Views

1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

Tags

Agrobacterium Tumefaciens

-- Views

Related Videos

article

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils

article

Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Generation of Patient-Derived Podocytes from Skin Biopsies

article

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

Generation and Manipulation of Rat Intestinal Organoids

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved