Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
22.7K Views
•
10:28 min
•
February 14th, 2020
DOI :
February 14th, 2020
•Alicia Masters1,2, Minjeong Kang1,3,4, Morgan McCaw1,3, Jacob D. Zobrist1,3,5, William Gordon-Kamm2, Todd Jones2, Kan Wang1,3
1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University
Chapters in this video
0:05
Introduction
1:01
Growing Donor Plants and Harvesting Immature Ears
2:40
Embryo Dissection, Infection, and Co-cultivation
5:54
Selection, Heat Treatment, and Regeneration
7:32
Greenhouse Transplantation and T1 Seed Production
8:08
Results: Representative Agrobacterium-mediated Immature Embryo Transformation
9:27
Conclusion
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