JoVE Logo
Faculty Resource Center

Sign In

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

DOI :

10.3791/2656-v

8:08 min

April 22nd, 2011

April 22nd, 2011

9,371 Views

1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Administration TVHS

We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.

Tags

Modified Mouse Embryonic Stem Cell Assay

-- Views

Related Videos

article

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

article

Derivation of Human Embryonic Stem Cells by Immunosurgery

article

Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research

article

Isolation and Derivation of Mouse Embryonic Germinal Cells

article

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

article

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

article

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

article

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

article

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

article

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved