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Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

DOI :

10.3791/2656-v

8:08 min

April 22nd, 2011

April 22nd, 2011

9,378 Views

1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Administration TVHS

We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.

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Modified Mouse Embryonic Stem Cell Assay

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