JoVE Logo
Faculty Resource Center

Sign In

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

DOI :

10.3791/55456-v

March 22nd, 2017

March 22nd, 2017

7,289 Views

1Department of Internal Medicine- Cardiology, UT Southwestern Medical Center

This manuscript describes a step-by-step protocol for the generation and quantification of diverse reprogrammed cardiac subtypes using a retrovirus-mediated delivery of Gata4, Hand2, Mef2c, and Tbx5.

Tags

Cardiomyocyte Subtypes

-- Views

Related Videos

article

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

article

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

article

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

article

Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

article

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

article

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

article

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

article

Dissection and Culture of Mouse Embryonic Kidney

article

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

article

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved