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Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

DOI :

10.3791/57687-v

June 3rd, 2018

June 3rd, 2018

7,157 Views

1Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia, Augusta University

Here we present a robust method to reprogram primary embryonic fibroblasts into functional cardiomyocytes through overexpression of GATA4, Hand2, Mef2c, Tbx5, miR-1, and miR-133 (GHMT2m) alongside inhibition of TGF-β signaling. Our protocol generates beating cardiomyocytes as early as 7 days post-transduction with up to 60% efficiency.

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Keywords Pro fibrotic Signaling

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