Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

18.9K Views

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13:21 min

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August 18th, 2012

DOI :

10.3791/2810-v

August 18th, 2012

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Bhavani Gopalakrishnan¹, Kevin M. Nash¹, Murugesan Velayutham¹, Frederick A. Villamena¹^,²

¹The Davis Heart and Lung Research Institute, The Ohio State University, ²Department of Pharmacology, College of Medicine, The Ohio State University

Summary

Chapters in this video

0:05

Title

0:09

Introduction

2:56

Detection of Nitric Oxide (NO) with Culture of Bovine Aortic Endothelial Cells (BAEC)

5:56

Endothelial NOS (eNOS) Uncoupling Experiment using SIN-1

8:12

Detection of superoxide (O₂^-) from polymorphonuclear neutrophils (PMNs)

10:20

Conclusion

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