January 11th, 2019
•Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.
Related Videos
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
In Vivo Calcium Imaging in C. elegans Body Wall Muscles
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Microcrystal Electron Diffraction of Small Molecules
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved