JoVE Logo
Faculty Resource Center

Sign In

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays (Video) | JoVE

DOI :

10.3791/2960-v

September 26th, 2011

September 26th, 2011

31,031 Views

1The virology Core at the HIV Drug Resistance Program, NCI-Frederick, 2Division of Infectious Diseases, University of Pittsburgh, 3Department of Molecular Biology and Microbiology, Tuffts University

Quantifying levels of HIV-1 RNA in plasma and sequencing single HIV-1 genomes from individuals with viral loads below the limit of detection (50-75 copies/ml) is difficult. Here we describe how to extract and quantify plasma viral RNA using a real time PCR assay that reliably measures HIV-1 RNA down to 0.3 copies/ml and how to amplify viral genomes by single genome sequencing, from samples with very low viral loads.

-- Views

Related Videos

article

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3 (Video) | JoVE

article

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays (Video) | JoVE

article

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach (Video) | JoVE

article

In vitro Uncoating of HIV-1 Cores (Video) | JoVE

article

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers Video (Video) | JoVE

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals Video (Video) | JoVE

article

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System. Video (Video) | JoVE

article

Nucleocapsid Annealing-Mediated Electrophoresis NAME Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors Video (Video) | JoVE

article

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals Video (Video) | JoVE

article

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1 Video (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved