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Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells Video (Video) | JoVE

DOI :

10.3791/58715-v

13:07 min

January 30th, 2019

January 30th, 2019

8,832 Views

1Department of Infectious Diseases, School of Immunology & Microbial Sciences, King's College London

Here we present a deep sequencing approach that provides an unbiased determination of nascent 3'-termini as well as mutational profiles of single-stranded DNA molecules. The main application is the characterization of nascent retroviral complementary DNAs (cDNAs), the intermediates generated during the process of retroviral reverse transcription.

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3 Termini

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