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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

DOI :

10.3791/58016-v

10:18 min

October 16th, 2018

October 16th, 2018

11,285 Views

1Centre for Virus Research, The Westmead Institute for Medical Research, University of Sydney

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

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Amplification

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