JoVE Logo
Faculty Resource Center

Sign In

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

DOI :

10.3791/3060-v

10:21 min

September 21st, 2011

September 21st, 2011

42,074 Views

1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.

Tags

Automated Hydrophobic Interaction Chromatography

-- Views

Related Videos

article

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

article

Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41

article

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

article

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

article

Purification of Hsp104, a Protein Disaggregase

article

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

article

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

article

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

article

Identifying Protein-protein Interaction Sites Using Peptide Arrays

article

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved