JoVE Logo
Faculty Resource Center

Sign In

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

DOI :

10.3791/62075-v

May 21st, 2021

May 21st, 2021

3,790 Views

1School of Life Sciences, University of Liverpool, 2Health Sciences Center, Texas Tech University

A multi-column plate adapter allows chromatography columns to be interfaced with multi-well collection plates for parallel affinity or ion exchange purification providing an economical high throughput protein purification method. It can be used under gravity or vacuum yielding milligram quantities of protein via affordable instrumentation.

-- Views

Related Videos

article

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

article

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

article

Author Spotlight: Unraveling the Role of Earthworms in Enhancing Mineral Weathering for CO2 Removal

article

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

article

Cloning of Solute Carriers (SLCs) into pHTBV1.1 Bacmid for High-Throughput Protein Expression

article

Author Spotlight: Advancing Protein Structure Analysis for Drug Development

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved