November 10th, 2011
•The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
Related Videos
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion
Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
Chromatin Isolation by RNA Purification (ChIRP)
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy
Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved