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In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

DOI :

10.3791/3138-v

12:35 min

November 10th, 2011

November 10th, 2011

18,604 Views

1Department of Biochemistry, University of Nebraska, Lincoln

The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.

Tags

In Vivo Liver Endocytosis

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