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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

DOI :

10.3791/53069-v

March 30th, 2016

March 30th, 2016

35,689 Views

1Department of General-, Visceral- and Transplantation Surgery, Charité University Medicine Berlin, 2Brandenburg Center for Regenerative Therapies, Charité University Medicine Berlin

A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.

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Keywords Primary Human Hepatocytes

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