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An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

DOI :

10.3791/61812-v

October 25th, 2021

October 25th, 2021

7,702 Views

1Department of Pediatrics, Johns Hopkins University School of Medicine, 2Department of Physiology, Temple University School of Medicine, 3Department of Pediatrics, Seattle Children’s Hospital

Primary hepatocytes are a valuable tool to study liver response and metabolism in vitro. Utilizing commercially available reagents, an improved time- and labor-efficient protocol for mouse primary hepatocyte isolation was developed.

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Mouse Primary Hepatocyte Isolation

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