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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

DOI :

10.3791/3410-v

January 27th, 2012

January 27th, 2012

14,090 Views

1Department of Cell and Molecular Biology, Karolinska Institutet

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.

Tags

Kinase

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