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Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

DOI :

10.3791/62241-v

April 22nd, 2021

April 22nd, 2021

2,398 Views

1Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, 2Gynecologic Oncology Division, Stanford University School of Medicine

Förster Resonance Energy Transfer (FRET) between two fluorophore molecules can be used for studying protein interactions in the living cell. Here, a protocol is provided as to how to measure FRET in live cells by detecting sensitized emission of the acceptor and quenching of the donor molecule using confocal laser scanning microscopy.

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