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Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

DOI :

10.3791/58619-v

November 2nd, 2018

November 2nd, 2018

7,969 Views

1Department of Biomedical Engineering, Duke University

Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.

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