JoVE Logo
Faculty Resource Center

Sign In

A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells

DOI :

10.3791/3616-v

April 12th, 2012

April 12th, 2012

15,082 Views

1Biozentrum, University of Basel

Cultured muscle cells are an inadequate model to recapitulate innervated muscle in vivo. A functional motor unit can be reproduced in vitro by innervation of differentiated human primary muscle cells using rat embryo spinal cord explants. This article describes how co-cultures of spinal cord explants and muscle cells are established.

Tags

Functional Motor Unit

-- Views

Related Videos

article

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

article

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

article

Chicken Embryo Spinal Cord Slice Culture Protocol

article

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

article

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

article

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

article

Laminectomy and Spinal Cord Window Implantation in the Mouse

article

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

article

Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord

article

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved