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In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

DOI :

10.3791/61396-v

6:01 min

December 3rd, 2020

December 3rd, 2020

4,940 Views

1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

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In Vitro

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