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Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices

DOI :

10.3791/62959-v

10:48 min

September 7th, 2021

September 7th, 2021

3,946 Views

1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute, KU Leuven - University of Leuven, 2VIB Bio Imaging Core, KU Leuven - University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology, KU Leuven - University of Leuven, 4Department of Neurology, University Hospitals Leuven

We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.

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