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Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays

DOI :

10.3791/3691-v

March 23rd, 2012

March 23rd, 2012

25,278 Views

1Department of Neuroscience & Pharmacology, Rudolf Magnus Institute for Neuroscience, University Medical Center Utrecht

Explants from the midbrain dopamine system and striatum are used in a collagen matrix assay for the in vitro analysis of mesostriatal and striatonigral pathway development. In this assay axonal outgrowth and guidance can be manipulated and quantified. It can also be modified for assessing other regions or molecular cues.

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Dissection

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