JoVE Logo
Faculty Resource Center

Sign In

Primary Culture of Mouse Dopaminergic Neurons

DOI :

10.3791/51751-v

September 8th, 2014

September 8th, 2014

36,356 Views

1CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, 2Inserm, U661, Montpellier, 3Universités de Montpellier

Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

Tags

Mouse Dopaminergic Neurons

-- Views

Related Videos

article

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

article

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

article

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

article

Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays

article

Calcium Phosphate Transfection of Primary Hippocampal Neurons

article

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

article

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

article

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

article

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

article

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved