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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

DOI :

10.3791/3747-v

11:58 min

February 29th, 2012

February 29th, 2012

82,546 Views

1MRC Centre for Synaptic Plasticity, University of Bristol

This report describes the use of live cell imaging and photobleach techniques to determine the surface expression, transport pathways and trafficking kinetics of exogenously expressed, pH-sensitive GFP-tagged proteins at the plasma membrane of neurons.

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Lateral Diffusion

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