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JoVE Journal

Neuroscience

This content is Open Access.

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

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11:58 min

February 29th, 2012

February 29th, 2012

82,799 Views

0:05

Title

1:33

Cell Culture, Viral Transduction, and Protein Expression

2:16

FRAP-FLIP Live Cell Imaging: Equipment Set-up and Image Capture Parameters

5:48

FRAP-FLIP Live Cell Imaging: Image Acquisition

7:09

Data Analysis

9:25

Representative FRAP-FLIP Results

11:34

Conclusion

This report describes the use of live cell imaging and photobleach techniques to determine the surface expression, transport pathways and trafficking kinetics of exogenously expressed, pH-sensitive GFP-tagged proteins at the plasma membrane of neurons.

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