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Measuring Exocytosis in Neurons Using FM Labeling

DOI :

10.3791/117-v

November 30th, 2006

November 30th, 2006

15,699 Views

1Department of Molecular and Cell Biology, Harvard

The ability to measure the kinetics of vesicle release can help provide insight into some of the basics of neurotransmission. Here we used real-time imaging of vesicles labeled with the red fluorescent dye FM 4-64 to measure the rate of presynaptic vesicle release in hippocampal neuronal cultures.

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