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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

DOI :

10.3791/3822-v

13:08 min

September 9th, 2012

September 9th, 2012

18,643 Views

1Applied Bioscience Program, Faculty of Science, University of Ontario Institute of Technology , 2Nursing Program, Faculty of Health Sciences, University of Ontario Institute of Technology , 3Medical Laboratory Science Program, Faculty of Health Sciences, University of Ontario Institute of Technology

This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.

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Factor V Activity

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