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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

DOI :

10.3791/3916-v

May 28th, 2012

May 28th, 2012

33,805 Views

1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

We describe a modified hot aqueous-phenol extraction method for purifying lipopolysaccharide (LPS) from Gram-negative bacteria. Once extracted, the LPS can be subsequently analyzed by SDS-PAGE and visualized by direct staining or Western immunoblot.

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Purification

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