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Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

DOI :

10.3791/59538-v

May 15th, 2019

May 15th, 2019

9,418 Views

1Department of Microbiology and Molecular Genetics, Michigan State University, 2Department of Physiology, Michigan State University

This method provides a framework for studying incorporation of exogenous fatty acids from complex host sources into bacterial membranes, particularly Staphylococcus aureus. To achieve this, protocols for the enrichment of lipoprotein particles from chicken egg yolk and subsequent fatty acid profiling of bacterial phospholipids utilizing mass spectrometry are described.

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Keywords Lipoprotein Particles

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