JoVE Logo
Faculty Resource Center

Sign In

Ganglioside Extraction, Purification and Profiling

DOI :

10.3791/62385-v

March 12th, 2021

March 12th, 2021

3,852 Views

1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 2Department of Neuroscience, Johns Hopkins University School of Medicine

Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

Tags

Ganglioside Extraction

-- Views

Related Videos

article

Laboratory Scale Production and Purification of a Therapeutic Antibody

article

Expression, Purification, and Antimicrobial Activity of S100A12

article

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

article

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

article

Expression and Purification of Mammalian Bestrophin Ion Channels

article

Extraction and Analysis of Taiwanese Green Propolis

article

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

article

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

article

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

article

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved