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Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

DOI :

10.3791/4052-v

September 5th, 2012

September 5th, 2012

11,722 Views

1Section of Neurobiology, Center for Learning and Memory, The University of Texas at Austin

Understanding the function of the vertebrate central nervous system requires recordings from many neurons because cortical function arises on the level of populations of neurons. Here we describe an optical method to record suprathreshold neural activity with single-cell and single-spike resolution, dithered random-access scanning. This method records somatic fluorescence calcium signals from up to 100 neurons with high temporal resolution. A maximum-likelihood algorithm deconvolves the underlying suprathreshold neural a

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Optical Recording

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