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Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

DOI :

10.3791/57015-v

January 19th, 2018

January 19th, 2018

7,345 Views

1Department of Genetics and Evolution, University of Geneva

The goal of this protocol is to establish ex vivo Drosophila larval brain culture optimized to monitor circadian molecular rhythms with long-term fluorescence time-lapse imaging. The application of this method to pharmacological assays is also discussed.

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Keywords Single cell

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