A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
22.1K Views
•
12:22 min
•
December 30th, 2012
DOI :
December 30th, 2012
•Explore More Videos
Chapters in this video
0:05
Title
2:44
Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide
4:13
Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes
5:43
Treatment of Infected Macrophages with Test Drugs
6:43
Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect
8:09
Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages
8:59
Quantitative Analysis of Transformed Promastigotes
9:22
Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug
10:41
Conclusion
Related Videos
Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
21.8K Views
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
7.6K Views
Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
12.2K Views
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
8.5K Views
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
6.5K Views
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
10.4K Views
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11.8K Views
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
9.6K Views
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
26.5K Views
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
8.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved