Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue

23.0K Views

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10:11 min

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January 3rd, 2013

DOI :

10.3791/4059-v

January 3rd, 2013

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Elaine Bigelow¹^,², Katherine M. Bever¹^,², Haiying Xu¹^,²^,³, Allison Yager¹, Annie Wu¹^,²^,⁴, Janis Taube³^,⁵, Lieping Chen⁶, Elizabeth M. Jaffee¹^,²^,⁵^,⁷^,⁸, Robert A. Anders¹^,²^,⁵^,⁸, Lei Zheng¹^,²^,⁴^,⁵^,⁷

¹The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, ²Department of Oncology, The Johns Hopkins University School of Medicine, ³Department of Dermatology, The Johns Hopkins University School of Medicine, ⁴Department of Surgery, Johns Hopkins University School of Medicine, ⁵The Sol Goldman Pancreatic Cancer Center, The Johns Hopkins University School of Medicine, ⁶Yale Cancer Center, Yale School of Medicine, ⁷The Skip Viragh Center for Pancreatic Cancer, The Johns Hopkins University School of Medicine, ⁸Department of Pathology, The Johns Hopkins University School of Medicine

Summary

Chapters in this video

0:05

Title

1:40

Preparing Paraffin-embedded Slides

2:52

Antigen Retrieval

3:39

Staining

7:53

Dehydration and Mounting

8:40

Immunohistochemical Stains

9:39

Conclusion

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