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Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

DOI :

10.3791/4061-v

September 12th, 2012

September 12th, 2012

14,676 Views

1The Cellular Neurobiology Unit, Centre de Recherche Université Laval Robert-Giffard

We describe a protocol for real-time videoimaging of neuronal migration in the mouse forebrain. The migration of virally-labeled or grafted neuronal precursors was recorded in acute live slices using wide-field fluorescent imaging with a relatively rapid acquisition interval to study the different phases of cell migration, including the durations of the stationary and migration phases and the speed of migration.

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Time lapse Imaging

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