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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

DOI :

10.3791/50989-v

11:32 min

November 12th, 2013

November 12th, 2013

11,153 Views

1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London

Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

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Nucleofection

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