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Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

DOI :

10.3791/409-v

November 1st, 2007

November 1st, 2007

35,028 Views

1Department of Physiology and Biophysics, University of California, Irvine (UCI)

Isolation of lymphocytes using the Miltenyi MACs kit is a reliable way to purify cells from whole lymphoid tissue homogenates. Cells purified using the Miltenyi system are typically ≥ 96% pure. Here, we demonstrate the steps taken to isolate CD4+ T cells, one of the many kits offered by Miltenyi.

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CD4 T Cells

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